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Electromagnetic field, a novel tool to
increase germination and seedling vigour of conserved onion (Allium
cepa L.) and rice (Oryza sativa L.) seeds with low
viability
M.P. Alexander¹ and S.D. Doijode²
¹ Magnetobiology Centre, 60, 3rd Cross, Jaibharath Nagar, Bangalore,
560 033, India ² Section of Plant Genetic Resources, Indian
Institute of Horticultural Research, Hessaraghatta, Bangalore, 560
089, India
Summary
Onion seed conserved for 5 years with a
reduced viability of only 41% was exposed to a static magnetic field
created by two identical bar magnets connected in a parallel
circuit. The magnets were placed one above the other with opposing
polarity with an air gap of 2.5 cm. Seeds were placed in the air gap
for pulsing with magnetic field strengths (MFS) of 36, 72, 108 and
144 Oersteds at doses of 30 and 60 minutes of exposure. The MFS of
108 Oersteds for 30 minutes significantly increased emergence,
germination of seeds, shoot and root lengths as well as fresh and
dry weights of the resultant seedlings. Rice seeds conserved for 6
years with the very low viability of 8.1% were exposed to an
alternating electromagnetic field (EMF) created by coupling a
function generator with a controlled magnetic field enclosure in a
series circuit. The seeds were exposed to a frequency of 5 Hz with
an amplitude of 1500 nanoTesla for 12 hours. Pulsing with the
alternating EMF significantly increased germination of seed, shoot
and root lengths, as well as the shoot/root ratio of seedlings. The
increase in germination, compared with the control, was 36.60% for
onion and 161.48% for rice. The significant increase in the factors
contributing to seedling vigour also showed that magnetic stimulus
was beneficial in conserving onion and rice seeds. Magnetic stimulus
could be used in genebanks to test the viability of conserved onion
and rice seeds about to be discarded because of low viability.
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Revised on April 03, 2006. Address comments on the
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