Cryostorage of in vitro-induced dormant buds of primula after desiccation

Satoshi Kato, Masaya Ishikawa, Miwako Ito and Tatsuo Matsumoto 338

Cryopreservation of oil-palm embryogenic suspensions

Nathalie Chabrillange, F. Aberlenc–Bertossi, M. Noirot, Y. Duval and Florent Engelmann 341

In vitro cryopreservation of cacao genetic resources

Bruno Florin, Eric Brulard and Vincent Pétiard 344

Development of a simplified method for the routine cryopreservation of coffee germplasm collection

Bruno Florin, Eric Brulard, Jean Paul Ducos, Hervé Tessereau and Vincent Pétiard 348

Incorporation of antibodies into tobacco cells treated with solutions similar to vitrification solutions

Pramod Tandon, Masaya Ishikawa, Atsushi Komanine and Hiroo Fukuda 352

Cryopreservation of Doritaenopsis cells in suspension culture by vitrification

H. Tsukazaki, Keiko Ishikawa and M. Mii 356

A cryopreservation protocol for strawberry cell suspension cultures

Yongjie Wu, Florent Engelmann, Andrea Frattarelli and Carmine Damiano 359

Current status of pollen cryopreservation research: relevance to tropical horticulture

S. Ganeshan and R.K. Rajashekaran 363

Cryopreservation of maize (Zea mays L.) pollen

Eiji Yamaguchi and Masaya Ishikawa 369

Cryopreservation of somatic embryos of sweet potato by slow prefreezing method

Kei Shimonishi, Minoru Karube and Masaya Ishikawa 371

Seeds of the African pepper bark (Warburgia salutaris) can be cryopreserved after rapid dehydration in silica gel

Joseph Kioko, Patricia Berjak, Hugh Pritchard and Matthew Daws 375

Effects of moisture content on Passiflora seed viability after immersion in liquid nitrogen

J.A. Ospina, C.L. Guevara, L.E. Caicedo and V. Barney 384

Cold acclimation improves the cryopreservation of in vitro-grown Pyrus and Rubus meristems

Yongjian Chang and Barbara M. Reed 389

Replacement of cold acclimatization with high sucrose pretreatment in black currant cryopreservation

Dominique Dumet, Yongjian Chang, Barbara M. Reed and Erica E. Benson 393

Structural observations on potato shoot-tips after thawing from liquid nitrogen

Ali M. Golmirzaie, Ana Panta and Cecilia Delgado 396

Cryopreservation of pineapple (Ananas comosus) apices by vitrification

María Teresa González–Arnao, Manfred Márquez Ravelo, Caridad Urra Villavicencio, Marcos E. Martínez Montero and Florent Engelmann 398

Research on cryopreservation of mango shoot-tips

Xue–Lin Huang, Jie–Ning Xiao, Xiao–Ju Li, Yun–Feng Cheng and Jia–Rui Fu 401

Cryopreservation of melon shoot primordia cultures using a slow prefreezing procedure

Rie Ito–Ogawa, Masaya Ishikawa, Eiko Niwata and Katsuji Oosawa 404

Survival of dried axillary buds of micropropagated redgrass

Manabu Katano and Yoshihiro Masuya 407

Cryopreservation of in vitro-grown shoot-tips of cassava cooled to –196°C by vitrification

R. Charoensub, S. Phansiri, Akira Sakai and W. Yongmanitchai 410

Cassava cryopreservation – I

Roosevelt H. Escobar, Graciela Mafla and William M. Roca 413

Cassava cryopreservation – II

Roosevelt H. Escobar, Juan D. Palacio, Maria P. Rangel and William M. Roca 417

Cryopreservation of shoot apices of yams Dioscorea (species) by vitrification

P.M. Kyesmu and Hiroko Takagi 420

Survival and recovery of yam (Dioscorea spp.) apices after cryopreservation using encapsulation-dehydration

Bernard Malaurie, Marie–France Trouslot, Nathalie Chabrillange and Florent Engelmann 423

Cryopreservation of cassava and yam shoot-tips by fast freezing

Shou Yong Choy Ng and Nyat Quat Ng 428

Cryopreservation of tissue-cultured tropical forest trees

Emilio Maruyama and Katsuaki Ishii 432

Cryopreservation of grape in vitro-cultured axillary shoot-tips by three-step vitrification

Toshikazu Matsumoto and Akira Sakai 435

Cryopreservation of shoot apices of lawngrass by encapsulation-dehydration

Tomohiro Minami and Akira Sawai 437

Cryopreservation of garlic apical meristems by vitrification

Eiko Niwata, Masahiko Suzuki and Masaya Ishikawa 440

Cryoexposure of in vitro shoot-tips of mangosteen – effects of sucrose and desiccation

M.N. Normah and B.S. Tan 443

Cryopreservation of almond apices using encapsulation-dehydration and vitrification

Mohamad A. Shatnawi, Florent Engelmann, Andrea Frattarelli and Carmine Damiano 446

Cryopreservation of shoot-tips of two leguminous trees (Acacia mangium and Paraserianthes falcataria) using encapsulation-dehydration and vitrification

Enny Sudarmonowati, Rosmithayani, E.S. Mulyaningsih, D. Priadi and Akira Sakai 449

Cryopreservation of in vitro-grown apical meristems of terrestrial orchids (Cymbidium spp.) by vitrification

Nguyen Tien Thinh and Hiroko Takagi 453

Cryopreservation of hairy root cultures of useful plants by encapsulation-dehydration

Kazumasa Hirata and Kazuhisa Miyamoto 456

Differential response and freezing tolerance of horticultural plant tissues by sugar-incubation in aid of cryopreservation

Yutaka Jitsuyama, T. Suzuki, T. Harada and Seizo Fujikawa 458

Cryopreservation at CATIE: an additional tool for the conservation of tropical agricultural crops and forest species

María Elena Aguilar, Nelly Vásquez, Emelda Yah, Florent Engelmann and François Côte 461

In vitro conservation and cryopreservation of papaya

Sarah Ashmore, Rodney Saunders and Roderick Drew 465

Cryopreservation of seeds, embryos, embryonic axes and pollen at National Cryobank of NBPGR

Rekha Chaudhury 470

Systematic determination of an adequate method for large-scale sweet potato cryopreservation at CIP

Ali M. Golmirzaie, Ana Panta and Sandra Diaz 473

Pilot plant cell culture collections cryopreserved in liquid nitrogen

Masaya Ishikawa, Tadashi Yokoyama and Kenichi Higo 476

Cryopreservation of some Malaysian tropical urban forestry species

M. Marzalina and Z.N.A. Nashatul 479

Present activities and perspectives on in vitro conservation in the MAFF Genebank, Japan

Susumu Miyashita, Yasufumi Kunihiro, Tsukasa Nagamine and Kazuto Shirata 483

Cryopreservation: an in vitro method for conserving Ribes germplasm in international genebanks

Barbara M. Reed, R.M. Brennan and Erica E. Benson 485

Cryopreservation of apple in vitro germplasm in China

Yongjie Wu, Florent Engelmann and Yanhua Zhao 488