FURTHER CHARACTERIZATION AND EVALUATION

5. SITE DATA
6. PLANT DATA
7. STRESS SUSCEPTIBILITY
8. PEST AND DISEASE SUSCEPTIBILITY
9. ALLOENZYME COMPOSITION
10. CYTOLOGICAL CHARACTERS AND IDENTIFIED GENES
11. NOTES
12. DESCRIPTORS OF NUTRITIONAL IMPORTANCE OTHER THAN UNDER 6.3
13. FUTURE DESCRIPTORS

5. SITE DATA

5.1 COUNTRY OF FURTHER CHARACTERIZATION AND EVALUATION

5.2 SITE (RESEARCH INSTITUTE)

5.3 NAME OF PERSON IN CHARGE OF EVALUATION

5.4 SOWING DATE

5.5 TRANSPLANTING DATE

5.6 FIRST HARVEST DATE

5.7 LAST HARVEST DATE

6. PLANT DATA

6.1 SEEDLING

Observe at 5 - leaf stage

6.1.1 Cotyledon length [mm]

See Fig. 10

6.1.2 Cotyledon width [mm]

See Fig. 10

6.1.3 Cotyledon notch depth

See Fig. 10

3 Shallow
5 Intermediate
7 Deep

Fig. 10. Cotyledon length and width, and notch depth

6.1.4 Hypocotyl colour

1 White
2 Greenish white
3 Light green
4 Green
5 Light purple green
6 Purple green
7 Purple
8 Other (specify in the NOTES descriptor, 11)

6.1.5 Anthocyanin pigment at seedling stage

0 Absent
1 Hypocotyl
2 Hypocotyl and petiole
3 Hypocotyl and petiole and blade
4 Other (specify in the NOTES descriptor, 11)

Observe immediately before harvest in non-flowering plants

6.2.1 Morphotype uniformity

After discarding obvious off-types

1 Uniform
2 Continuous variation
3 Two or more distinct types

6.2.2 Basal side shoot development

0 Absent
3 Sparse
5 Intermediate
7 Abundant

6.2.3 Weight of whole plant without root [g]

6.2.4 Number of non-wrapper leaves

6.2.5 Leaf length [cm]

See Fig. 11

Measure largest leaf

6.2.6 Leaf width [cm]

See Fig. 11

Measure widest point of largest leaf

6.2.7 Petiole length [cm]

See Fig. 11

Measure portion where leaf blade intercepts the petiole or is supposed to intercept, but not due to the presence of wing to petiole base of largest leaf

6.2.8 Petiole width [cm]

Measure widest point of petiole of largest leaf; midrib width in subspp. pekinensis and rapa

Fig. 11. Mature leaf length and width, and petiole length and width

6.2.9 Petiole thickness [mm]

See Fig. 12

Measure thickest point of petiole of largest leaf; midrib thickness in subspp. pekinensis and rapa

6.2.10 Cross-sectional view of petiole

See Fig. 12

Take the widest part of the midrib in subspp. pekinensis and rapa

3 Round
5 Semi - round
7 Flat

Fig. 12. Petiole thickness (PT) and cross-sectional view

6.2.11 Leaf blade thickness

3 Thin
5 Intermediate
7 Thick

6.2.12 Leaf blade blistering

0 No blistering
3 Slight
5 Intermediate
7 High

6.2.13 Leaf margin undulation

See Fig. 13

0 Absent
3 Low
5 Intermediate
7 High

Fig. 13. Leaf margin undulation

6.3 INFLORESCENCE. SILIQUE AND SEED

6.3.1 Bolting behaviour under natural conditions [%]

Without artificial vernalization

6.3.2 Petal length [mm]

See Fig. 4

6.3.3 Petal width [mm]

See Fig. 4

6.3.4 Silique length [cm]

Seed bearing portion only

See Fig. 14

6.3.5 Silique width [cm]

Seed bearing portion only

See Fig. 14

6.3.6 Length of beak [cm]

See Fig. 14

Fig. 14. Silique width and length, and length of beak

6.3.7 Number of mature seeds per silique

6.3.8 Dominant seed coat colour

After drying

1 Yellow
2 Red brown
3 Brown
4 Dull grey
5 Almost black
6 Other (specify in the NOTES descriptor, 11)

6.3.9 1000 seed weight [g]

According to International Seed Testing Association (ISTA) rules; 5-6% moisture content ‘wet weight’

6.3.10 Seed oil content

Analytical technique to be specified in NOTES descriptor, 11

Observe at harvest, head - forming type only

6.4.1 Head weight [g]

Non-wrapper leaves removed

6.4.2 Length of head [cm]

Non - wrapper leaves removed

6.4.3 Head diameter [cm]

Non - wrapper leaves removed

6.4.4 Axillary bud growth

Before trimming

3 Inconspicuous
5 Intermediate
7 Clear

6.4.5 Heading ability under high temperature

At mean temperature of 25°C or above

3 Poor
5 Intermediate
7 Excellent

Observe at harvest on enlarged root-forming types only

6.5.1 Root length [cm]

Storage portion

6.5.2 Root diameter [cm]

Largest point

6.5.3 Root weight [g]

7. STRESS SUSCEPTIBILITY

Scored under artificial and/or natural conditions on a 1-9 scale, where

3 Low susceptibility
5 Medium susceptibility
7 High susceptibility

7.1 LOW-TEMPERATURE INJURY

7.2 GROWTH RATE UNDER LOW TEMPERATURE

7.3 HIGH-TEMPERATURE INJURY

7.4 DROUGHT

7.5 FLOODING/WATERLOGGING/EXCESS SOIL MOISTURE

7.6 SOIL SALINITY

7.7 SOIL ACIDITY

7.8 MICROELEMENT DEFICIENCY

7.9 MICROELEMENT TOXICITY

7.10 TIPBURN

Internal rot

8. PEST AND DISEASE SUSCEPTIBILITY

Scored on a 1-9 scale, where

0 Immunity
3 Low susceptibility
5 Medium susceptibility
7 High susceptibility

9. ALLOENZYME COMPOSITION

This may prove to be a useful tool for identifying duplicate accessions

10. CYTOLOGICAL CHARACTERS AND IDENTIFIED GENES

11. NOTES

Give additional information where descriptor state is noted as “Other” as, for example, in descriptors 4.1.2, 4.2.2. 4.2.3, etc. Also include here any other relevant information

12. DESCRIPTORS OF NUTRITIONAL IMPORTANCE OTHER THAN UNDER 6.3

Certain characters which have or may have significant nutritional implication for food, animal feed and feed technology industries and whose assays are technically more demanding should be accumulated by genebanks or laboratories, e.g. erucic acid, glucosinolate, etc.

13. FUTURE DESCRIPTORS

The list will remain open for inclusion of future descriptors, such as those pertaining to physiological traits, which can be rapidly screened. Most of the characters in the present list are of a visual morphological nature and an appropriate extension would be to use microscopic evaluation of tissue distribution at critical sites. Fluorescent probes now exist for many compounds, e.g. H₂0, protein, lipids, starch, lignin, DNA, RNA, as well as specific probes, e.g. immunofluorescent protein probes. Distribution of many of these compounds is potentially important for stress resistance factors (lignin with lodging for example), quality factors (seed protein distribution) and yield (e.g. stem starch and vascular tissue development). These tissue distributions are genetically controlled and not subject to great environmental interaction